| Registro completo |
| Provedor de dados: |
Genet. Mol. Biol.
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| País: |
Brazil
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| Título: |
MLL leukemia-associated rearrangements in peripheral blood lymphocytes from healthy individuals
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| Autores: |
Brassesco,María Sol
Montaldi,Ana Paula
Gras,Diana Ester
Queiroz,Rosane Gomes de Paula
Martinez-Rossi,Nilce Maria
Tone,Luiz Gonzaga
Sakamoto-Hojo,Elza Tiemi
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| Data: |
2009-01-01
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| Ano: |
2009
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| Palavras-chave: |
Genomic instability
Lymphocytes
MLL rearrangements
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| Resumo: |
Chromosomal translocations are characteristic of hematopoietic neoplasias and can lead to unregulated oncogene expression or the fusion of genes to yield novel functions. In recent years, different lymphoma/leukemia-associated rearrangements have been detected in healthy individuals. In this study, we used inverse PCR to screen peripheral lymphocytes from 100 healthy individuals for the presence of MLL (Mixed Lineage Leukemia) translocations. Forty-nine percent of the probands showed MLL rearrangements. Sequence analysis showed that these rearrangements were specific for MLL translocations that corresponded to t(4;11)(q21;q23) (66%) and t(9;11) (20%). However, RT-PCR failed to detect any expression of t(4;11)(q21;q23) in our population. We suggest that 11q23 rearrangements in peripheral lymphocytes from normal individuals may result from exposure to endogenous or exogenous DNA-damaging agents. In practical terms, the high susceptibility of the MLL gene to chemically-induced damage suggests that monitoring the aberrations associated with this gene in peripheral lymphocytes may be a sensitive assay for assessing genomic instability in individuals exposed to genotoxic stress.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200005
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| Editor: |
Sociedade Brasileira de Genética
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| Relação: |
10.1590/S1415-47572009000200005
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| Formato: |
text/html
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| Fonte: |
Genetics and Molecular Biology v.32 n.2 2009
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| Direitos: |
info:eu-repo/semantics/openAccess
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